Optimizing the Western Blot Technique to Detect GAPDH in Rat Skeletal Muscle Tissue Samples
Start Date
7-8-2026 11:15 AM
End Date
7-8-2026 11:30 AM
Location
ALT 203
Abstract
The Western Blot technique is used to measure protein expression and to quantify your results. Our summer research consisted of refining a fluorescent Western blot protocol to detect the cytosolic protein glyceraldehyde-3-phosphate dehydrogenase, or GAPDH. Our next step will be to detect the sodium-potassium pump, which is found in the plasma membrane. Our long-term goal is to use this protocol to detect sialyltransferases and sialidases in rat skeletal muscle samples. We will continue to optimize this protocol to eventually use our NASA rat samples to detect proteins involved in muscle atrophy.
Optimizing the Western Blot Technique to Detect GAPDH in Rat Skeletal Muscle Tissue Samples
ALT 203
The Western Blot technique is used to measure protein expression and to quantify your results. Our summer research consisted of refining a fluorescent Western blot protocol to detect the cytosolic protein glyceraldehyde-3-phosphate dehydrogenase, or GAPDH. Our next step will be to detect the sodium-potassium pump, which is found in the plasma membrane. Our long-term goal is to use this protocol to detect sialyltransferases and sialidases in rat skeletal muscle samples. We will continue to optimize this protocol to eventually use our NASA rat samples to detect proteins involved in muscle atrophy.
