3D Printed Engineered Technologies for qRT-PCR Techniques
Start Date
April 2025
Location
2nd floor - Library
Abstract
Semiquantitative real-time polymerase chain reaction (qRT-PCR) is used to detect and quantify RNA to measure relative gene expression by using fluorescently labeled probes which become activated upon binding. qRT-PCR may be done on a 96-well plate allowing for analysis of multiple samples at once. Such a technique can be prone to errors while filling wells, leading to lost time and money. Despite efforts to find a solution to this problem, the solutions remain imperfect. A novel way this problem can be rectified is by utilizing computer software to design and produce a physical tool through 3D printing. This tool can be adjustable, produced simply, and saves otherwise wasted materials, and it has support indicating its’ ability to minimize total loading errors.
3D Printed Engineered Technologies for qRT-PCR Techniques
2nd floor - Library
Semiquantitative real-time polymerase chain reaction (qRT-PCR) is used to detect and quantify RNA to measure relative gene expression by using fluorescently labeled probes which become activated upon binding. qRT-PCR may be done on a 96-well plate allowing for analysis of multiple samples at once. Such a technique can be prone to errors while filling wells, leading to lost time and money. Despite efforts to find a solution to this problem, the solutions remain imperfect. A novel way this problem can be rectified is by utilizing computer software to design and produce a physical tool through 3D printing. This tool can be adjustable, produced simply, and saves otherwise wasted materials, and it has support indicating its’ ability to minimize total loading errors.
